Information-enriched characterization of cell populations. Morphological information and facts from photos can add to your richness of flow cytometry data. By way of example, the figure reveals an or else common apoptosis assay utilizing Annexin V and PI, incorporating cell imaging to characterize cells in Each and every population to reveal morphologically distinct attributes. These insights couldn't happen to be acquired from multiplex staining on your own.
Whether or not you’re a rookie or an expert, explore our sources to advance your research and know-how in flow cytometry.
(B) Uncooked 264.seven macrophages in many cases are annotated inaccurately with respect to particle rely because of their complexity and noncircular borders. The number of singlets available for Assessment increases when the user trains and employs a far more precise design.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing with a substantial-pace brightfield digicam for simultaneous high throughput flow cytometry and significant resolution brightfield imaging.
"A picture just isn't rather really worth one thousand dots, but it really really helps to see images. It is excellent to determine illustrations or photos that correlate with staining. Such as, AnnexinV-good cells which might be morphologically diverse than healthier cells, or CD14+ monocytes which have been more substantial and much more textured than CD3+/CD4+/CD14- lymphocytes.
Right here, an experienced consumer has gated singlets confidently. After analyzing the handbook singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate consists of a lot more than four% aggregates.
Pair your Attune flow cytometer using an autosampler for elevated workflow performance, and incorporate robotic integration for optimum throughput.
Reliable impression excellent even at high throughput Acoustic concentrating in addition to a significant-speed digital camera Merge to graphic these T cells persistently at lower or superior flow fees. Quickly adjust focus and digital camera configurations to meet experimental requirements.
Early indications of this contamination experienced to begin with been dismissed as particles. Backgating to discover new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also permits you to use morphological options to discover interesting subpopulations that will not be apparent from flow cytometry info alone.
People can look at party photographs in actual-time to confirm sample high-quality, optimize protocols, and record the morphology of each mobile populace recognized for analysis. Imaging seize also Positive aspects from acoustic concentrating, which really helps to posture the cells to ensure that a pointy, centered image is attained.
AI-pushed automated image Evaluation Together with the Attune Cytometric Software program interprets occasion capabilities into unique morphology parameters that could be coupled with common fluorescence and scatter parameters to reveal very reproducible population changes and distinctive subsets.
Application note: Automatic graphic Investigation reduces person-to-consumer variability in flow cytometry gating tactics
Crafted-in dimensionality reduction equipment amplified resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to confirm predicted inhabitants resolution in lysed full human blood. Person can see situations from Just about every cluster for Visible affirmation of mobile phenotypes.
No wash/no lyse leukocyte Examination with imaging. Cells have been acquired from 24-hour-old blood by dilution in 1 mM EDTA ( Specialist UX/UI allows end users to prepare AI models for expanded apps. (A) The “Practice Model” function delivers annotation equipment and genuine-time product teaching precision.
At the center of every Attune Flow Cytometer lies our innovative acoustic focusing know-how, which makes use of seem waves to meticulously align cells throughout the flow mobile. This progressive technique surpasses standard hydrodynamic procedures, resulting in more quickly processing speeds, Extraordinary facts top quality, and improved sample throughput.
The Attune Xenith cytometer is a substantial-parameter spectral analyzer, supplying both spectral here unmixing and standard flow capabilities for extensive and functional mobile Examination.
The Attune CytPix Flow Cytometer allows end users to visualize samples during acquisition, confirm the accuracy of gating techniques, and assist effects with responsible documentation for publication reasons.
What seemed to be a homogeneous inhabitants is definitely much more assorted—an insight that should be thought of when interpreting effects.